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Dnmt1 has an essential function despite the absence of CpG DNA methylation in the[...]
Acknowledgements
WGBS confirms the absence of CpG methylation in Tribolium eggs.
Expression of Dnmt1.
Knockdown via maternal RNAi affects development and fecundity.
Figure 1 Correlation between the proportion of unconverted cytosines that were observed in both replicates of the WGBS and the log fold coverage at each site (Spearman rank, rho = -0.
Table 1 Gene expression of Dnmt1 normalised over the expression of two housekeeping genes.
Dnmt1 knockdown disrupts embryonic development.
Figure 2 Phenotypic effects of maternal RNAi (a) Eclosion rate (±SEM) of female pupae after Dnmt1 RNAi treatment.
Discussion
Figure 3 Dnmt1 knockdown and embryonic development (a) Timeline of naïve embryonic development.
Methods
Model organism.
Whole genome bisulfite sequencing.
Gene Expression Analysis.
Knockdown via parental RNAi.
Fitness costs of the knockdown.
Embryonic development after maternal knockdown.
Statistical analysis.
Acknowledgements
Journal Article
Dnmt1 has an essential function despite the absence of CpG DNA methylation in the red flour beetle Tribolium castaneum
Place and Date of Creation
07/11/2018
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