Schulz, Nora Kristin Elisa; Wagner, Clara Isabel; Ebeling, Julia; Raddatz, Günter; Diddens-de Buhr, Maike F.; Lyko, Frank; Kurtz, Joachim: Dnmt1 has an essential function despite the absence of CpG DNA methylation in the red flour beetle Tribolium castaneum
Inhalt
- WGBS confirms the absence of CpG methylation in Tribolium eggs.
- Figure 1 Correlation between the proportion of unconverted cytosines that were observed in both replicates of the WGBS and the log fold coverage at each site (Spearman rank, rho = -0.
- Table 1 Gene expression of Dnmt1 normalised over the expression of two housekeeping genes.
- Figure 2 Phenotypic effects of maternal RNAi (a) Eclosion rate (±SEM) of female pupae after Dnmt1 RNAi treatment.
- Discussion
- Figure 3 Dnmt1 knockdown and embryonic development (a) Timeline of naïve embryonic development.
- Methods
- Model organism.
- Whole genome bisulfite sequencing.
- Gene Expression Analysis.
- Knockdown via parental RNAi.
- Fitness costs of the knockdown.
- Embryonic development after maternal knockdown.
- Statistical analysis.
- Acknowledgements