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Protein engineering of the aldoxime dehydratase from Bacillus sp. OxB-1 based on[...]
Discussion
Assay design.
Identification of mutation sites.
Generation of the mutant library and initial screening.
Activity determination of the purified OxdB variants.
Mass spectrometric analysis.
Correlation between heme content and specific enzymatic activity.
Discussion
Conclusion
Methods
General information.
Identification of the mutation sites.
Generation of the mutant library.
Protein expression and purification.
Activity assay.
Mass spectrometry.
Substrate and reference compound synthesis.
Synthesis of Z-phenylacetaldoxime.
Synthesis of phenylacetonitrile.
References
Acknowledgements
Aufsatz in einer Zeitschrift
Protein engineering of the aldoxime dehydratase from Bacillus sp. OxB-1 based on a rational sequence alignment approach
Entstehung
2021
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