Oike, Keiko; Sproß, Jens; Matsui, Daisuke; Asano, Yasuhisa; Gröger, Harald: Protein engineering of the aldoxime dehydratase from Bacillus sp. OxB-1 based on a rational sequence alignment approach. In: Scientific reports. Jg.11 H. 1. 2021
Inhalt
- Assay design.
- Identification of mutation sites.
- Generation of the mutant library and initial screening.
- Activity determination of the purified OxdB variants.
- Mass spectrometric analysis.
- Correlation between heme content and specific enzymatic activity.
- Discussion
- Conclusion
- Methods
- General information.
- Identification of the mutation sites.
- Generation of the mutant library.
- Protein expression and purification.
- Activity assay.
- Mass spectrometry.
- Substrate and reference compound synthesis.
- References
- Acknowledgements