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Two Novel, Flavin-Dependent Halogenases from the Bacterial Consortia of Botryococcus[...]
Materials and Methods
Analytics
High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS)
Results and Discussion
Identification and Analysis of Novel, Flavin-Dependent Halogenases from the Botryococcus braunii Consortia
In Vitro Experiments with the Flavin-Dependent Halogenases SpH1 and SpH2
Determination of Halogenation Activity and Substrate Scope
Investigation of Mono- and Dibromination Activity
Materials and Methods
Analytics
Analytical, Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
Preparative, Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS)
Gas Chromatography–Mass Spectrometry (GC-MS)
Nuclear Magnetic Resonance (NMR) Spectroscopy
Bioinformatic Analysis
Metagenomic Analysis for the Detection of Flavin-Dependent Halogenases
Phylogenetic Analysis
In Vitro Enzyme Assays
Vector Construction and Molecular Cloning
Heterologous Protein Expression and Purification
Enzymatic Halogenation/Enzyme Assay with Purified Protein on an Analytical Scale
Determination of Specific Activity
Catalase Activity Assay
Enzymatic Halogenation with Immobilised Protein on a Preparative Scale
FAD Reconstitution
In Silico Study Using YASARA
Conclusions
References
Aufsatz in einer Zeitschrift
Two Novel, Flavin-Dependent Halogenases from the Bacterial Consortia of Botryococcus braunii Catalyze Mono- and Dibromination
Entstehung
2021
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