Neubauer, Pia R.; Blifernez-Klassen, Olga; Pfaff, Lara; Ismail, Mohamed; Kruse, Olaf; Sewald, Norbert: Two Novel, Flavin-Dependent Halogenases from the Bacterial Consortia of Botryococcus braunii Catalyze Mono- and Dibromination. In: Catalysts. Jg.11 H. 4. 2021
Inhalt
- Results and Discussion
- Identification and Analysis of Novel, Flavin-Dependent Halogenases from the Botryococcus braunii Consortia
- In Vitro Experiments with the Flavin-Dependent Halogenases SpH1 and SpH2
- Materials and Methods
- Analytics
- Analytical, Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
- Preparative, Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
- High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS)
- Gas Chromatography–Mass Spectrometry (GC-MS)
- Nuclear Magnetic Resonance (NMR) Spectroscopy
- Bioinformatic Analysis
- In Vitro Enzyme Assays
- Vector Construction and Molecular Cloning
- Heterologous Protein Expression and Purification
- Enzymatic Halogenation/Enzyme Assay with Purified Protein on an Analytical Scale
- Determination of Specific Activity
- Catalase Activity Assay
- Enzymatic Halogenation with Immobilised Protein on a Preparative Scale
- FAD Reconstitution
- In Silico Study Using YASARA
- Conclusions
- References