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Coenzyme Q10 biosynthesis established in the non-ubiquinone containing Corynebacterium[...]
Results
Metabolic Engineering for Improved Supply of Aromatic Precursor pHBA
Materials and Methods
Bacterial Strains and Growth Conditions
Recombinant DNA Work and Strain Construction
Quantification of Amorphadiene
Crude Extract Preparation and SDS–PAGE
LC-MS/MS Analysis of Ubi Proteins
Quantification of Metabolites via HPLC
Identification of CoQ10 by HPLC-ESI-MS and NanoESI-Q-TOF-MS
Bioreactor Fermentation of UBI413
Results
Functional Expression of DPP Synthase Gene ddsA From P. denitrificans in C. glutamicum
Metabolic Engineering for Improved Supply of Isoprenoid Precursor FPP
Metabolic Engineering for Improved Supply of Aromatic Precursor pHBA
Functional Expression of Polyprenyl Transferase Gene ubiA From E. coli
Functional Expression of Genes for the Ubiquinone Modification Pathway
CoQ10 Biosynthesis by Metabolically Engineered C. glutamicum
Discussion
Data Availability Statement
Author Contributions
Funding
Acknowledgments
Supplementary Material
References
Aufsatz in einer Zeitschrift
Coenzyme Q10 biosynthesis established in the non-ubiquinone containing Corynebacterium glutamicum by metabolic engineering
Entstehung
2021
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