Burgardt, Arthur; Moustafa, Ayham; Persicke, Marcus; Sproß, Jens; Patschkowski, Thomas; Risse, Joe Max; Peters-Wendisch, Petra; Lee, Jin-Ho; Wendisch, Volker F.: Coenzyme Q10 biosynthesis established in the non-ubiquinone containing Corynebacterium glutamicum by metabolic engineering. In: Frontiers in Biotechnology and Bioengineering. Jg.9. 2021
Inhalt
- Materials and Methods
- Bacterial Strains and Growth Conditions
- Recombinant DNA Work and Strain Construction
- Quantification of Amorphadiene
- Crude Extract Preparation and SDS–PAGE
- LC-MS/MS Analysis of Ubi Proteins
- Quantification of Metabolites via HPLC
- Identification of CoQ10 by HPLC-ESI-MS and NanoESI-Q-TOF-MS
- Bioreactor Fermentation of UBI413
- Results
- Functional Expression of DPP Synthase Gene ddsA From P. denitrificans in C. glutamicum
- Metabolic Engineering for Improved Supply of Isoprenoid Precursor FPP
- Metabolic Engineering for Improved Supply of Aromatic Precursor pHBA
- Functional Expression of Polyprenyl Transferase Gene ubiA From E. coli
- Functional Expression of Genes for the Ubiquinone Modification Pathway
- CoQ10 Biosynthesis by Metabolically Engineered C. glutamicum
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References