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A bottom-up approach towards a bacterial consortium for the biotechnological conversion[...]
Results
Simultaneous growth of substrate converter and producer strains on a mixture of acetate[...]
Bacterial strains and growth experiments
Preparation of colloidal chitin, GlcNAc2 and GlcN2
Preparation of cell-free supernatant of E.coli strains
Construction of E.coli strains
Determination of enzyme activities
Purification of enzymes
Determination of acetate concentrations
Statistics
Accession numbers
Results
Introducing metabolic deficiencies into substrate converter strain and producer strain
Simultaneous growth of substrate converter and producer strains on a mixture of acetate and glucosamine
Testing functionality of the chitin deacetylase secreted by the substrate converter
Co-culture and lysine cross feeding of substrate converter and producer strains on GlcNAc
Co-culture and lysine cross-feeding of substrate converter and producer strains on colloidal chitin with added or heterologously expressed chitinolytic enzymes
Discussion
References
Aufsatz in einer Zeitschrift
A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine
Entstehung
2021
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