Vortmann, Marina; Stumpf, Anna K.; Sgobba, Elvira; Dierks-Hofmeister, Mareike E.; Krehenbrink, Martin; Wendisch, Volker F.; Philipp, Bodo; Moerschbacher, Bruno M.: A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine. In: Applied Microbiology and Biotechnology. Jg.105. 2021, S. 1547-1561
Inhalt
- Bacterial strains and growth experiments
- Preparation of colloidal chitin, GlcNAc2 and GlcN2
- Preparation of cell-free supernatant of E.coli strains
- Construction of E.coli strains
- Determination of enzyme activities
- Purification of enzymes
- Determination of acetate concentrations
- Statistics
- Accession numbers
- Results
- Introducing metabolic deficiencies into substrate converter strain and producer strain
- Simultaneous growth of substrate converter and producer strains on a mixture of acetate and glucosamine
- Testing functionality of the chitin deacetylase secreted by the substrate converter
- Co-culture and lysine cross feeding of substrate converter and producer strains on GlcNAc
- Co-culture and lysine cross-feeding of substrate converter and producer strains on colloidal chitin with added or heterologously expressed chitinolytic enzymes
- Discussion
- References