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Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Met[...]
UV-Vis Absorption Spectroscopy and Turbidity Are Initial Approaches for Detecting[...]
Principles and General Considerations
UV-Vis Absorption Spectroscopy and Turbidity Are Initial Approaches for Detecting the Self-Assembly/Aggregation-Prone Tendency of Proteins
Principles and General Considerations
Indirect Methods: Evaluating Protein Absorption Spectra
Intrinsic Chromophores
Extrinsic Chromophores
Direct Methods: Analyzing Protein Aggregation in Solution
Fluorescence Spectroscopy Is a Versatile Tool for Evaluating Peptide and Protein Self-Assembly
Principles and General Considerations
Intrinsic Fluorophore
Extrinsic Fluorophore
Assessment of Protein and Peptide Self-Assembly Processes by Fluorescence Spectroscopy: Methods and Selected Examples
Steady-State Fluorescence Methods
Time-Resolved Methods
Fluorescence Correlation Spectroscopy (FCS)
Circular Dichroism Makes It Possible to Follow Secondary and Tertiary Structure Changes Due to Self-Assembly
Main Principles and Structural Protein Features
Experimental Considerations of CD Spectroscopy
Uses of Far-UV CD Spectroscopy on the Study of Self-Assembled Systems
Analysis of Amyloidogenic Proteins
Evaluation of Non-Amyloid Systems
The Use of CD to Assess Conformational Equilibriums during Self-Assembly by Changes on the Microenvironment
Near-UV CD Analysis of Protein Aggregation
Some Considerations in Order to Avoid Protein Aggregation
Conclusions
References
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Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
Entstehung
2020
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