Pignataro, Maria Florencia; Herrera, Maria Georgina; Dodero, Veronica Isabel: Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods. In: Molecules. Vol.25 Issue 20. 2020
Content
- UV-Vis Absorption Spectroscopy and Turbidity Are Initial Approaches for Detecting the Self-Assembly/Aggregation-Prone Tendency of Proteins
- Principles and General Considerations
- Indirect Methods: Evaluating Protein Absorption Spectra
- Direct Methods: Analyzing Protein Aggregation in Solution
- Fluorescence Spectroscopy Is a Versatile Tool for Evaluating Peptide and Protein Self-Assembly
- Principles and General Considerations
- Intrinsic Fluorophore
- Extrinsic Fluorophore
- Assessment of Protein and Peptide Self-Assembly Processes by Fluorescence Spectroscopy: Methods and Selected Examples
- Circular Dichroism Makes It Possible to Follow Secondary and Tertiary Structure Changes Due to Self-Assembly
- Main Principles and Structural Protein Features
- Experimental Considerations of CD Spectroscopy
- Uses of Far-UV CD Spectroscopy on the Study of Self-Assembled Systems
- Analysis of Amyloidogenic Proteins
- Evaluation of Non-Amyloid Systems
- The Use of CD to Assess Conformational Equilibriums during Self-Assembly by Changes on the Microenvironment
- Near-UV CD Analysis of Protein Aggregation
- Some Considerations in Order to Avoid Protein Aggregation
- Conclusions
- References