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Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of[...]
Methods
Overproduction and purification of RamB
Results
Growth of C. glutamicum WT and ΔphoRS on different phosphorus sources and under Pi limiting conditions
Deletion analysis of the pstS promoter
Identification of RamB as a protein binding to the pstS promoter
Purified RamB binds to two binding motifs in the pstS promoter invitro
Role of RamB sites for regulation of the pstS promoter invivo
Comparison of Pi starvation inducible gene expression on glucose and acetate minimal medium
Discussion
Conclusions
Methods
Bacterial strains, media, and growth conditions
Preparation of supernatants and assay to determine UDP-glucose hydrolase activity
Construction of transcriptional fusions and chloramphenicol acetyltransferase (CAT) assays
DNA affinity chromatography
MALDI-TOF mass spectrometry
Overproduction and purification of RamB
Gel mobility shift assays
DNA microarray analysis
Competing interests
Authors' contributions
Acknowledgements
References
Aufsatz in einer Zeitschrift
Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB
Entstehung
2015
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