Sorger-Herrmann, Ulrike; Taniguchi, Hironori; Wendisch, Volker F.: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. In: BMC Microbiology. Jg.15 H. 1. 2015
Inhalt
- Results
- Growth of C. glutamicum WT and ΔphoRS on different phosphorus sources and under Pi limiting conditions
- Deletion analysis of the pstS promoter
- Identification of RamB as a protein binding to the pstS promoter
- Purified RamB binds to two binding motifs in the pstS promoter invitro
- Role of RamB sites for regulation of the pstS promoter invivo
- Comparison of Pi starvation inducible gene expression on glucose and acetate minimal medium
- Discussion
- Conclusions
- Methods
- Bacterial strains, media, and growth conditions
- Preparation of supernatants and assay to determine UDP-glucose hydrolase activity
- Construction of transcriptional fusions and chloramphenicol acetyltransferase (CAT) assays
- DNA affinity chromatography
- MALDI-TOF mass spectrometry
- Overproduction and purification of RamB
- Gel mobility shift assays
- DNA microarray analysis
- Competing interests
- Authors' contributions
- Acknowledgements
- References