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Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin[...]
Bacterial strains, media and growth conditions
Promoter exchange of the dxs gene in C. glutamicum LYC3
Bacterial strains, media and growth conditions
Recombinant DNA work
Deletion of carotenogenic genes in C. glutamicum MB001
Construct design of the synthetic MEP operons and their integration into the genome of C. glutamicum LYC3
Promoter exchange of the dxs gene in C. glutamicum LYC3
Overexpression of carotenogenic genes
Extraction analysis of carotenoids
DXS activity assay
Results
Overexpression of dxs increased lycopene yield
Overproduction of enzymes converting DXP to IPP using two synthetic operons integrated into the C. glutamicum chromosome
Improved IPP supply by chromosome-based enhancement of MEP pathway gene expression
Application of C. glutamicum with improved IPP supply for production of decaprenoxanthin and astaxanthin
Discussion
References
Aufsatz in einer Zeitschrift
Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum
Entstehung
2014
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