Heider, Sabine; Wolf, Natalie; Hofemeier, Arne; Peters-Wendisch, Petra; Wendisch, Volker F.: Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. In: Frontiers in Bioengineering and Biotechnology. Jg.2. 2014
Inhalt
- Bacterial strains, media and growth conditions
- Recombinant DNA work
- Deletion of carotenogenic genes in C. glutamicum MB001
- Construct design of the synthetic MEP operons and their integration into the genome of C. glutamicum LYC3
- Promoter exchange of the dxs gene in C. glutamicum LYC3
- Overexpression of carotenogenic genes
- Extraction analysis of carotenoids
- DXS activity assay
- Results
- Overexpression of dxs increased lycopene yield
- Overproduction of enzymes converting DXP to IPP using two synthetic operons integrated into the C. glutamicum chromosome
- Improved IPP supply by chromosome-based enhancement of MEP pathway gene expression
- Application of C. glutamicum with improved IPP supply for production of decaprenoxanthin and astaxanthin
- Discussion
- References