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Improvement of the design and generation of highly specific plant knockdown lines[...]
Results
Phenotypic analyses of transgenic lines expressing pri-smiRNA (CHS)
Results
Design of synthetic primary-microRNAs (pri-smiRNAs)
Molecular analyses of transgenic lines expressing pri-smiRNA (CHS)
Phenotypic analyses of transgenic lines expressing pri-smiRNA (CHS)
Off-target analysis
Design of smiRNAs: mismatches and excess G:U base pairs
Discussion
Conclusion
Methods
Design of synthetic microRNAs
Plant material and growth conditions
Agrobacterium-mediated transformation of Arabidopsis thaliana
Small RNA Northern blots
Analysis of secondary metabolites in Arabidopsis thaliana
Quantitative RT-PCR experiments
Target Validation experiments
Acknowledgements
Author details
Authors' contributions
Competing interests
References
Aufsatz in einer Zeitschrift
Improvement of the design and generation of highly specific plant knockdown lines using primary synthetic microRNAs
(pri-smiRNAs)
Entstehung
2010
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