Niemeier, Sandra; Alves jun., Leonardo; Merkle, Thomas: Improvement of the design and generation of highly specific plant knockdown lines using primary synthetic microRNAs (pri-smiRNAs). In: BMC Research Notes. Jg.3 H. 1. 2010
Inhalt
- Results
- Design of synthetic primary-microRNAs (pri-smiRNAs)
- Molecular analyses of transgenic lines expressing pri-smiRNA (CHS)
- Phenotypic analyses of transgenic lines expressing pri-smiRNA (CHS)
- Off-target analysis
- Design of smiRNAs: mismatches and excess G:U base pairs
- Discussion
- Conclusion
- Methods
- Design of synthetic microRNAs
- Plant material and growth conditions
- Agrobacterium-mediated transformation of Arabidopsis thaliana
- Small RNA Northern blots
- Analysis of secondary metabolites in Arabidopsis thaliana
- Quantitative RT-PCR experiments
- Target Validation experiments
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References