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Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel[...]
References
Background
Results
Effective pH homeostasis is correlated with optimal growth in C. glutamicum
Transcriptome and proteome analyses of pH acclimatization in C. glutamicum
Iron homeostasis of C. glutamicum is affected by the external pH
At neutral and acidic pH H2O2 can be detected in C. glutamicum cultures
Metabolic alterations during response to acidic pH
The McbR regulon is induced at acidic pH
Differential expression of further regulatory modules
Discussion
At neutral and low pH C. glutamicum is impaired by oxidative stress
The occurrence of oxidative stress interferes with iron availability and control of metabolic fluxes in C. glutamicum
Cysteine accumulation inhibits the cysthationine-b-lyase AecD and causes additional limitations for growth of C. glutamicum under acidic conditions
Conclusions
Methods
Strains and culture conditions
Determination of bioenergetic parameters
Detection and elimination of H2O2, protein carbonylation and cysthationine lyase activity
Transcriptome analysis
Proteome analysis
Metabolome analysis
Authors' contributions
Additional material
Acknowledgements
References
Aufsatz in einer Zeitschrift
Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis
Entstehung
2009
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