Follmann, Martin; Ochrombel, Ines; Kraemer, Reinhard; Troetschel, Christian; Poetsch, Ansgar; Rückert, Christian; Hueser, Andrea; Persicke, Marcus; Seiferling, Dominic; Kalinowski, Jörn; Marin, Kay: Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine [...]. In: BMC Genomics. Jg.10 H. 1. 2009, S. 621 ff.
Inhalt
- Background
- Results
- Effective pH homeostasis is correlated with optimal growth in C. glutamicum
- Transcriptome and proteome analyses of pH acclimatization in C. glutamicum
- Iron homeostasis of C. glutamicum is affected by the external pH
- At neutral and acidic pH H2O2 can be detected in C. glutamicum cultures
- Metabolic alterations during response to acidic pH
- The McbR regulon is induced at acidic pH
- Differential expression of further regulatory modules
- Discussion
- At neutral and low pH C. glutamicum is impaired by oxidative stress
- The occurrence of oxidative stress interferes with iron availability and control of metabolic fluxes in C. glutamicum
- Cysteine accumulation inhibits the cysthationine-b-lyase AecD and causes additional limitations for growth of C. glutamicum under acidic conditions
- Conclusions
- Methods
- Strains and culture conditions
- Determination of bioenergetic parameters
- Detection and elimination of H2O2, protein carbonylation and cysthationine lyase activity
- Transcriptome analysis
- Proteome analysis
- Metabolome analysis
- Authors' contributions
- Additional material
- Acknowledgements
- References