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The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene[...]
Conclusion
Results
Differences of salt-dependent expression of SAPK4 in rice and in F. rubra ssp. litoralis
Over-expression of SAPK4 in transgenic rice plants
SAPK4 is involved in tolerance to salt stress in rice
SAPK4 regulates ion accumulation in salt-stressed rice
Discussion
Conclusion
Methods
Plant material, growth conditions, and salt stress
Extraction of RNA, Northern hybridization, hybridization of cDNA-arrays, and RT-PCR
Construction of subtraction cDNA-library
Preparation of cDNA-macroarrays, labeling of probes, and hybridization of cDNA-arrays
Generation of constructs and transformation of rice
Isolation of protoplasts and subcellular localization of SAPK4-GFP fusion proteins
Measurement of chlorophyll a fluorescence and of Cl- content
Authors' contributions
Acknowledgements
References
Aufsatz in einer Zeitschrift
The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice
Entstehung
2008
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