Diedhiou, Calliste J.; Popova, Olga V.; Dietz, Karl-Josef; Golldack, Dortje: The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. In: BMC PLANT BIOLOGY. Jg.8 H. 1. 2008, S. 49 ff.
Inhalt
- Results
- Differences of salt-dependent expression of SAPK4 in rice and in F. rubra ssp. litoralis
- Over-expression of SAPK4 in transgenic rice plants
- SAPK4 is involved in tolerance to salt stress in rice
- SAPK4 regulates ion accumulation in salt-stressed rice
- Discussion
- Conclusion
- Methods
- Plant material, growth conditions, and salt stress
- Extraction of RNA, Northern hybridization, hybridization of cDNA-arrays, and RT-PCR
- Construction of subtraction cDNA-library
- Preparation of cDNA-macroarrays, labeling of probes, and hybridization of cDNA-arrays
- Generation of constructs and transformation of rice
- Isolation of protoplasts and subcellular localization of SAPK4-GFP fusion proteins
- Measurement of chlorophyll a fluorescence and of Cl- content
- Authors' contributions
- Acknowledgements
- References