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Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA[...]
ACKNOWLEDGMENTS
RESULTS
Crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase reactions in M. sedula cell extracts.
Characterization of an enoyl-CoA hydratase Msed_0566.
Quantification of enoyl-CoA hydratase homologs in autotrophically grown M. sedula.
Msed_2001 is a promiscuous 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase.
Characterization of 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase Nmar_1308 from N. maritimus and its phylogenetic analysis.
Characterization of a putative crotonyl-CoA hydratase Slip_2089 from S. lipocalidus.
DISCUSSION
MATERIALS AND METHODS
Materials.
Microbial strains and growth conditions.
CoA-esters synthesis.
Preparation of M. sedula cell extracts.
Gene cloning.
Heterologous expression in E. coli.
Purification of recombinant proteins.
Enzyme assays.
Database search and phylogenetic analysis.
Mass spectrometric expression analysis.
Other methods.
Data availability.
SUPPLEMENTAL MATERIAL
ACKNOWLEDGMENTS
REFERENCES
Journal Article
Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Place and Date of Creation
20/01/2021
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