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Autodisplay for the co-expression of lipase and foldase on the surface of E. coli:[...]
Results:
Construction of the plasmid for autodisplay of lipase
Surface display of lipase
Construction of the plasmid for autodisplay of foldase
Surface display of foldase
Coexpression of both LipBc-FP and FoldBc-FP
Lipase Activity of whole cells co-expressing LipBc-FP and FoldBc-FP
Lipase activity of outer membrane preparations from E. Coli BL21(DE3) pAT-LiFoBc
Application of cells and membrane preparations in a standardized laundry test
Discussion
Conclusion
Methods
Bacterial strains, plasmids and culture conditions
Recombinant DNA techniques
Outer membrane protein preparation
SDS-PAGE
Flow cytometer analysis
Lipase activity assay
Laundry tests with lipase-whole cell biocatalyst/ E. coli BL21(DE3) pAT-LipBc
Competing interests
Authors’ contributions
Acknowledgements
Author details
References
Aufsatz in einer Zeitschrift
Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs
Entstehung
29.1.2014
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