Kranen, Eva Elisabeth; Detzel, Christian; Weber, Thomas; Jose, Joachim: Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs
Inhalt
- Results:
- Construction of the plasmid for autodisplay of lipase
- Surface display of lipase
- Construction of the plasmid for autodisplay of foldase
- Surface display of foldase
- Coexpression of both LipBc-FP and FoldBc-FP
- Lipase Activity of whole cells co-expressing LipBc-FP and FoldBc-FP
- Lipase activity of outer membrane preparations from E. Coli BL21(DE3) pAT-LiFoBc
- Application of cells and membrane preparations in a standardized laundry test
- Discussion
- Conclusion
- Methods
- Bacterial strains, plasmids and culture conditions
- Recombinant DNA techniques
- Outer membrane protein preparation
- SDS-PAGE
- Flow cytometer analysis
- Lipase activity assay
- Laundry tests with lipase-whole cell biocatalyst/ E. coli BL21(DE3) pAT-LipBc
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References