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A Replication-Competent HIV Clone Carrying GFP-Env Reveals Rapid Env Recycling at[...]
Results
Imaging HIV-1 Carrying Fluorescent Env Constructs
Materials and Methods
Cell Lines and Virus Production
Human Primary CD4 T Cells
Viruses
p24 ELISA
Western Blot Analysis
Infectivity TZM-bl Assay
Cell-to-Cell Transfer Assay
Growth Curve of HIV Env-V4.2-sfGFP
Fluorescence Microscopy Sample Preparation
Confocal and Live Imaging
Fluorescence Recovery after Photobleaching (FRAP)
Super-Resolution Optical Microscopy of HIV-Infected T Cells
Results
Engineering an Infectious HIV Carrying a sfGFP Insertion into the Env V4 or V5 Domains
Imaging HIV-1 Carrying Fluorescent Env Constructs
A dual Fluorescent Protein-Expressing HIV with Gag-iCherry and Env-sfGFP Participates in VS-Mediated HIV Transfer
Method for Pulse-Chase Labeling of Surface Env Tracks Endocytosis and Relocalization to the VS
Fluorescence Recovery after Photobleaching (FRAP) of HIV Env V4.2 sfGFP-Gag-iCherry at VS Reveals Constitutive Turnover of Env near the VS
High Turnover of Env Proximal to the VS Requires Endocytosis of Env Using a Membrane Proximal Tyrosine Y712
Discussion
References
Aufsatz in einer Zeitschrift
A Replication-Competent HIV Clone Carrying GFP-Env Reveals Rapid Env Recycling at the HIV-1 T Cell Virological Synapse
Entstehung
2022
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