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Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain[...]
Acknowledgments
2 Materials and Methods
2.1 Bacterial Strains, Plasmids and Culture Media
2.2 DNA Isolation, Transfer and Manipulation
2.3 Construction of Plasmids
2.4 Site-specific Gene Disruption/Replacement
2.5 Transposon Mutant Sequencing
2.6 Determination of Growth Rates and Transformation Efficiency
2.7 Genome Sequencing, Assembly, and Accession Numbers
3 Results
3.1 Construction of Strain CR101
3.2 Development of a Genetic Switch Based System for Random Insertion Mutagenesis
3.3 Sequencing of ML103 Transposon Mutants
4 Discussion
Data Availability Statement
Author Contributions
Acknowledgments
Supplementary Material
References
Aufsatz in einer Zeitschrift
Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase
Entstehung
2021
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