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Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by[...]
Results
Nanopore Long-Read Amplicon Sequencing Identified a Large Deletion in ORF8 in an[...]
Materials and Methods
RNA Extraction and RT-qPCR Assays
Nanopore Sequencing
Illumina Sequencing
Sanger Sequencing
Bioinformatic and Data Analysis
Phylogenetic and ORF8 Deletion Analyses
Structural Computational Analysis of ORF8
Results
Nanopore Long-Read Amplicon Sequencing Identified a Large Deletion in ORF8 in an Infection Chain from a Local Hospital
The Same 168 Bases Long Deletion Has Occurred Independently throughout Europe
Standard Amplicon Sequencing and Variant Calling Pipelines Potentially Hide Other Large Deletions
Discussion
References
Journal Article
Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines
Place and Date of Creation
2021
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