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Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases[...]
Materials and Methods
Musa FLS1 and Musa FLS3
Materials and Methods
Plant Material
Phylogenetic Analysis
Expression Analysis
Total RNA Extraction, cDNA Synthesis and Molecular Cloning
Heterologous Expression in E. coli
F3H and FLS Bioconversion Assay in E. coli
Agrobacterium -Mediated Transformation of A. thaliana
Flavonoid Staining of A. thaliana Seedlings
Analysis of Flavonols in Methanolic Extracts
Determination of Anthocyanin Content
Results
Creation of cDNA Constructs and Sequence-Based Characterisation of Musa Flavonoid 2-ODDs
Expression Profiles of Musa 2-ODD Genes
Musa F3H1 and Musa F3H2 Are Functional F3H
Musa FLS1 and Musa FLS3 Are Functional FLS
Musa ANS Is a Functional ANS
Discussion
Musa F3H1 and Musa F3H2
Musa FLS1 and Musa FLS3
Musa ANS
Data Availability Statement
Author Contributions
Supplementary Material
References
Aufsatz in einer Zeitschrift
Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis
Entstehung
2021
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