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Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting[...]
Materials and Methods
Plasmids and Molecular Cloning
Expression and Purification of Recombinant Thal-RebH5
Crystallization and Data Collection
Data Processing, Structure Determination and Refinement
Accession Numbers
Results
Crystallization Details and Comparison to Thal-RebH5
Comparison of the Previously Described P64 and the New P21 Crystal Forms
Crystal Contacts and Possible Impact of the His-tag
Discussion
Differing Functional Regions
Impact of VM on Diffraction
Impact of His-tag on Crystallization
References
Aufsatz in einer Zeitschrift
Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals
Entstehung
2020
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