Moritzer, Ann-Christin; Prior, Tina; Niemann, Hartmut: Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. In: Crystals. Jg.10 H. 12. 2020
Inhalt
- Materials and Methods
- Plasmids and Molecular Cloning
- Expression and Purification of Recombinant Thal-RebH5
- Crystallization and Data Collection
- Data Processing, Structure Determination and Refinement
- Accession Numbers
- Results
- Crystallization Details and Comparison to Thal-RebH5
- Comparison of the Previously Described P64 and the New P21 Crystal Forms
- Crystal Contacts and Possible Impact of the His-tag
- Discussion
- References