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Sequence-Specific DNA Binding by Noncovalent Peptide–Azocyclodextrin Dimer Complex[...]
Materials and Methods
Synthesis and Characterization of azoCyDdimer
Introduction
Conformational Fuzziness and Fuzzy Complexes
Design of the Non-Covalent Externally Controlled Biomimetic System
Results
Synthesis and Characterization of the Allosteric Receptor and Adamantyl Substituted Peptides
Allosteric Receptor Synthesis and Characterization
Peptide Derivatives Synthesis
Peptide–dsDNA Interaction
Electrophoretic Mobility Shift Assays (EMSA)
Circular Dichroism Spectroscopy
Competitive Binding Assay with 1-Adamantane Acetic Acid by CD and EMSA Experiments
Discussion
Materials and Methods
Peptide Synthesis
Synthesis and Characterization of azoCyDdimer
Annealing of dsDNA
Electrophoretic Shift binding Assays
Circular Dichroism Spectroscopy B17-molecules-518593
References
Aufsatz in einer Zeitschrift
Sequence-Specific DNA Binding by Noncovalent Peptide–Azocyclodextrin Dimer Complex as a Suitable Model for Conformational Fuzziness
Entstehung
2019
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