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RNA-seq assembly - are we there yet?
References
Considerations for NGS Transcriptome Assembly
Assessing the Assembly
Number of unigenes
Number of reference transcripts matching the unigenes
Number of reference transcripts hit by reads compared to number of reference transcripts hit by unigenes
N50, average length, median length
Length of the longest unigene
Number of estimated full length unigenes
Number of hybrid/read-through unigenes
Example Workflow
Read cleaning (Supplemental Script 1 lines 6–11)
Assembly (Supplemental Script 1 lines 13–27)
Mapping (Supplemental Script 1 lines 29–46)
Quality assessment (Supplemental Script 1 lines 48–87)
Final polish of the assembly (Supplemental Scripts 1 lines 89–99)
Applying the workflow (quick and dirty)
Conclusion
Supplementary Material
References
Aufsatz in einer Zeitschrift
RNA-seq assembly - are we there yet?
Entstehung
2012
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