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SMRT sequencing only de novo assembly of the sugar beet (Beta vulgaris) chloroplast[...]
Conclusions
Methods
Plant material and DNA extraction
Library construction and sequencing
Raw sequencing data
Extraction of potential chloroplast reads
Assembly
Creation of coverage plots
Validating, reordering and polishing the assembly
Annotation of the assembly
Comparing the assemblies
Results and discussion
Subread extraction
Result of the assembly process
Comparison to the available sugar beet chloroplast assembly
Annotation
Conclusions
Availability of supporting data
Additional files
Abbreviations
Competing interests
Authors’ contributions
Authors' information
Acknowledgements
Author details
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SMRT sequencing only de novo assembly of the sugar beet (Beta vulgaris) chloroplast genome
Entstehung
2015
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