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Fast forward genetics to identify mutations causing a high light tolerant phenotype[...]
Background
Results
Generation and phenotypical characterization of high light tolerant C. reinhardtii mutants
Genotypical characterization of hit1 and hit2 by whole genome re-sequencing
Confirmation of the causative mutation in LRS1 referring to HL tolerance
Localization of the mutations in a predicted WD40 ß-propeller motif of LRS1
Discussion
Whole genome sequencing to identify a gene involved in high light adaptation in C. reinhardtii
Identification of LRS1 reveals new perspectives for the characterization of regulatory mechanisms for high light adaptation in microalgae
Conclusion
Methods
Chlamydomonas strains and mutant generation
Mutant phenotype characterization
Whole genome sequencing and identification of unique mutations
Backcrossing and segregation analysis
In silico analysis of LRS1
Availability of supporting data
Abbreviations
Competing interests
Authors’ contributions
Acknowledgements
Author details
References
Aufsatz in einer Zeitschrift
Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing
Entstehung
2015
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