Schierenbeck, Lisa; Ries, David; Rogge, Kristin; Grewe, Sabrina; Weisshaar, Bernd; Kruse, Olaf: Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing. In: BMC Genomics. Jg.16 H. 1. 2015
Inhalt
- Background
- Results
- Generation and phenotypical characterization of high light tolerant C. reinhardtii mutants
- Genotypical characterization of hit1 and hit2 by whole genome re-sequencing
- Confirmation of the causative mutation in LRS1 referring to HL tolerance
- Localization of the mutations in a predicted WD40 ß-propeller motif of LRS1
- Discussion
- Whole genome sequencing to identify a gene involved in high light adaptation in C. reinhardtii
- Identification of LRS1 reveals new perspectives for the characterization of regulatory mechanisms for high light adaptation in microalgae
- Conclusion
- Methods
- Chlamydomonas strains and mutant generation
- Mutant phenotype characterization
- Whole genome sequencing and identification of unique mutations
- Backcrossing and segregation analysis
- In silico analysis of LRS1
- Availability of supporting data
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References