de
en
Schliessen
Detailsuche
Bibliotheken
Projekt
Impressum
Datenschutz
Detailsuche
Schnellsuche:
OK
Ergebnisliste
Titel
Titel
Inhalt
Inhalt
Seite
Seite
Im Dokument suchen
A novel type of N-acetylglutamate synthase is involved in the first step of arginine[...]
Discussion
In vivo and invitro experiments confirm the ability of Cg3035 to acetylate l-glu[...]
Results
Discovering a gene responsible for the first step of arginine biosynthesis
Validating the gene function of cg3035 by heterologous complementation, gene deletion, metabolome analyses, and enzyme assays
Cg3035 establishes a novel class of NAGS genes
Discussion
Metabolite profiling analyses provide new insight into arginine biosynthesis
In vivo and invitro experiments confirm the ability of Cg3035 to acetylate l-glutamate
Cg3035 – A novel form of N-acetylglutamate synthase
Conclusions
Methods
Bacterial strains, growth conditions, plasmids, and oligonucleotides
DNA isolation, manipulation, and analysis
Construction of defined chromosomal deletions
Construction of expression vectors
Generation of a genomic library from C. glutamicum ATCC 13032 and complementation of E. coli JM109 ∆argA
Cell harvesting, metabolite extraction, and sample preparation
Sample preparation for qualitative LC-MS analysis
Sample preparation for quantitative LC-MS analysis
HPLC-ESI-QTOF conditions
Processing of LC-MS(/MS) Data
Preparation of crude protein extracts of bacterial cultures
Measurement of NAGS enzyme activity
Additional files
Competing interests
Authors' contributions
Acknowledgements & funding
References
Aufsatz in einer Zeitschrift
A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum
Entstehung
2013
JPEG-Download
verfügbare Breiten
JPEG groß
JPEG original
Seiten an
Seiten aus
