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Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based[...]
Conclusion
Background
Results
Transposon mutagenesis of Corynebacterium glutamicum ATCC 13032 with an IS6100-based transposon vector
Auxanographic screening and targeted gene identification using PCR techniques
Identification of a novel C. glutamicum gene closing the last gap in histidine biosynthesis
Validation of cg0910 gene function by deletion and homologous complementation
Phylogenetic analysis of Cg0910 and related inositol monophosphatases (IMP)
Discussion
Conclusion
Methods
Bacterial strains, plasmids, oligonucleotides and culture conditions
DNA isolation, manipulation and transfer
Transformation of C. glutamicum
Determination of transposon integration sites
Sequence data/accession numbers
Bioinformatic analyses and tools for interpretation of C. glutamicum sequences
Auxotrophy screening and supplementation of auxotrophic phenotypes
PCR techniques and conditions
Deletion and genetic complementation of cg0910
Authors' contributions
Acknowledgements
References
Aufsatz in einer Zeitschrift
Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway
Entstehung
2006
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