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Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection[...]
Background
Results and discussion
Annotation of sequences from the ESTs library
Validation of best reference genes for qRT-PCR
Conclusion
Methods
Balanus amphitrite, culturing and RNA extraction
EST library creation and sequencing
Clustering, assembly and functional annotation of the EST library
Primer design
cDNA synthesis, primer efficiencies and cycle parameter for qRT-PCR
qRT-PCR data analysis
Authors' contributions
Additional material
Acknowledgements
References
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Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies
Entstehung
2009
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