De Gregoris, Tristano Bacchetti; Borra, Marco; Biffali, Elio; Bekel, Thomas; Burgess, J. Grant; Kirby, Richard R.; Clare, Anthony S.: Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. In: BMC Molecular Biology. Jg.10 H. 1. 2009, S. 62 ff.
Inhalt
- Background
- Results and discussion
- Conclusion
- Methods
- Balanus amphitrite, culturing and RNA extraction
- EST library creation and sequencing
- Clustering, assembly and functional annotation of the EST library
- Primer design
- cDNA synthesis, primer efficiencies and cycle parameter for qRT-PCR
- qRT-PCR data analysis
- Authors' contributions
- Additional material
- Acknowledgements
- References