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  • Title
    stochprofML: Stochastic Profiling Using Maximum Likelihood Estimation in R
  • Author
    Amrhein, Lisa ; Fuchs, Christiane
  • Is part of
    BMC Bioinformatics, Vol. 22
  • Published
    2021
  • Language
    English
  • Document type
    Journal Article
  • URN
    urn:nbn:de:0070-pub-29447355 
  • DOI
    10.1186/s12859-021-03970-7 
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Abstract
  • Background

    Tissues are often heterogeneous in their single-cell molecular expression, and this can govern the regulation of cell fate. For the understanding of development and disease, it is important to quantify heterogeneity in a given tissue.
    Results

    We present the R package stochprofML which uses the maximum likelihood principle to parameterize heterogeneity from the cumulative expression of small random pools of cells. We evaluate the algorithm’s performance in simulation studies and present further application opportunities.
    Conclusion

    Stochastic profiling outweighs the necessary demixing of mixed samples with a saving in experimental cost and effort and less measurement error. It offers possibilities for parameterizing heterogeneity, estimating underlying pool compositions and detecting differences between cell populations between samples.
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