Kerbs, Anastasia; Mindt, Melanie; Schwardmann, Lynn; Wendisch, Volker F.: Sustainable Production of N-methylphenylalanine by Reductive Methylamination of Phenylpyruvate Using Engineered Corynebacterium glutamicum. In: Microorganisms. Jg.9 H. 4. 2021
Inhalt
- Materials and Methods
- Bacterial Strains and Growth Conditions
- Molecular Genetic Techniques and Strain Construction
- Protein Analytics
- Quantification of Amino Acids and Organic Acids
- Results
- Assessing the Suitability of C. glutamicum for NMePhe Production
- Exchanging Two Amino Acid Residues of DpkA to Affect Preference of the Substrates Pyruvate and Phenylpyruvate
- Metabolic Engineering of C. glutamicum for Efficient Provision of Phenylpyruvate as Precursor
- DpkA Mediated Methylamination of Phenylpyruvate Yielded NMePhe
- Improvement of NMePhe Production and Reduction of NMeAla
- Establishing NMePhe Production from the Alternative Feedstock Xylose
- Discussion
- References