Feiner, Rebecca; Kemker, Isabell; Krutzke, Lea; Allmendinger, Ellen; Mandell, Daniel J.; Sewald, Norbert ; Kochanek, Stefan; Müller, Kristian: EGFR-Binding Peptides: From Computational Design towards Tumor-Targeting of Adeno-Associated Virus Capsids. In: International Journal of Molecular Sciences. Jg.21 H. 24. 2020
Inhalt
- Results
- Computational Design of -Hairpin Peptides Competitively Inhibiting Dimerization of EGFR
- Synthesis and Cyclization Of Peptides
- Biophysical Characterization of Peptides
- Cyclic Peptides Show Binding to Cells Expressing EGFR at High and Low Levels
- Cyclic Peptide Pep1osy Shows Inhibitory Properties in Wound Healing Assays
- Peptides Grafted on the Recombinant Adeno-Associated Virus 2 Capsid
- Insertion of Designed EGFR-Targeting Peptides Changes the Tropism of rAAV2 towards EGFR Overexpressing Cells
- rAAV2 587 Pep1jhf Demonstrates Efficacy in Egg Xenografts
- Discussion
- Materials and Methods
- Structural Modeling
- General Procedure
- Peptide Synthesis
- N-Terminal Fluorescein Labeling
- Peptide Cleavage
- Cyclization of Peptides
- Cell Culture
- Wound Healing Assay
- Confocal Fluorescence Microscopy
- Circular Dichroism Spectrometry
- Fluorescence Polarization
- Viral Particle Production
- Purification of Viral Particles
- SDS-PAGE and Western Blot Analysis
- Determination of Genomic Titers
- Transduction Assay
- Chorioallantoic Membrane (CAM) Assay
- References