Wolter, Steve: An accurate and efficient algorithm for real-time localisation of photoswitchable fluorophores. 2009
Inhalt
- Introduction
- Photoswitching microscopy overview
- Temporal confinement and deconfinement
- Motivation for and goal of present work
- Structure of this Thesis
- Theory of photoswitching microscopy
- Algorithmic theory
- Implementation of a deconfinement program
- State of the Art
- Specification
- The rapidSTORM engine
- The rapidSTORM image construction
- Guessing the spot standard deviation
- Experimental material and methods
- dSTORM image acquisition process
- dSTORM stacks selected for testing
- Stochastically generating data sets
- Measuring error rates in candidate search stage
- Computational hard- and software
- Results and discussion
- Evaluation by comparison with Schüttpelz implementation
- Candidate search
- Spot fitting and judging stage
- estimator
- Real-time computation
- Conclusion and Outlook
- Credits
- Proof of recursion formula for sum of squares
- Schematic overview over rapidSTORM system
- Tables
- Schüttpelz implementation