Soluble brain homogenates from diverse human and mouse sources preferentially seed diffuse Aβ plaque pathology when injected into newborn mouse hosts. In: Free Neuropathology. Jg.3. 23.3.2022
Inhalt
- Abbreviations
- Background
- Methods
- Description of AD, PA and NDC cases
- Transgenic mice
- Human brain lysates
- Brain seeding with neonatal cerebral ventricle injections
- Mouse brain tissue collection
- Histology and immunochemistry
- Brain amyloid extraction
- ELISA
- Contextual fear conditioning
- Results
- Characterization of AD/LBD, AD, PA, and NDC cases
- Cerebral injection of brain lysates into newborn APP/tau and APP mice results in widespread, robust amyloid deposition
- Seeding diffuse Aβ deposition does not impair performance in a fear-conditioning cognitive task
- Assessment of Aβ seeding efficiency in APPsi mice
- Discussion
- Conclusions
- Ethics approval
- Consent for publication
- Availability of data and materials
- Competing interests
- Funding
- Authors’ contributions
- Acknowledgements
- Conflict of interest