Teixeira, Felipe C. O. B.; Kumar, Archana Vijaya; Katakam, Sampath Kumar; Cocola, Cinzia; Pelucchi, Paride; Graf, Monika; Kiesel, Ludwig; Reinbold, Rolland; Pavão, Mauro S. G.; Greve, Burkhard; Götte, Martin: The Heparan Sulfate Sulfotransferases HS2ST1 and HS3ST2 Are Novel Regulators of Breast Cancer Stem-Cell Properties
Inhalt
- Materials and Methods
- Cell Lines and Reagents
- CD24 and CD44 Identification With Flow Cytometry
- Identification of ALDH-1 Positive Cells
- Colony Formation Assay
- Analysis of Spheroid Size by the Hanging Drop Method
- Mammosphere Assay
- Quantitative Real-Time PCR
- Western Blotting
- Statistical Analysis
- Results
- HS2ST1 and HS3ST2 Overexpression in MDA-MB-231 and MCF-7 Cells Alter the Expression of the CSC Markers CD24 and CD44, and ALDH1 Enzymatic Activity
- HS2ST1 and HS3ST2 Overexpression Modifies Colony Formation, and Number and Size of MDA-MB-231 and MCF-7 Tumor Spheres
- The Overexpression of HS2ST1 and HS3ST2 Regulates the Expression of Factors Related to EMT and Stem Cell Function
- The Syndecan Family of HSPGs and the Notch Pathway Are Part of the Regulatory Circuit of HS Sulfotransferases
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References