Börschel, Christin S.; Stejskalová, Anna; Schäfer, Sebastian Daniel; Kiesel, Ludwig; Götte, Martin: miR-142-3p Reduces the Size, Migration, and Contractility of Endometrial and Endometriotic Stromal Cells by Targeting Integrin- and Rho GTPase-Related [...]
Inhalt
- Methods
- Cell Culture
- microRNA Transfection
- qRT-PCR
- DUAL-Luciferase Reporter Assay
- Western Blot
- Cell Spread Area Analysis
- Migration Assay
- Collagen Gel Contraction Assay
- Immunofluorescence Staining
- Imaging
- Bioinformatics
- Statistical Analysis
- Results
- In Silico Analysis Predicts miRNA-142-3p Targets Several Rho GTPases and Integrin Signaling Pathway Constituents
- qRT-PCR Validates miR-142-3p Regulates ROCK2, CFL2, RAC1 and WASL Gene Expression Levels
- Luciferase Validation of The Predicted Targets WASL and ITGAV
- Protein-Level Validation of WASL and ITGAV
- miR-142-3p Lowers the Concentration of Vinculin in Focal Adhesions
- Functional Analysis of the Role of miRNA-142-3p in Endometriosis Suggests Its Effects on Endometrial Stromal Cell Size, Migration, and Collagen I Contractility
- Discussion
- Conclusions
- References