Heinemann, Luca; Möllers, Klara Maria; Mohammed Ahmed, Helal Mohammed; Wei, Lanying ; Sun, Kaiyan; Nimmagadda, Subbaiah Chary; Frank, Daria; Baumann, Anja; Poos, Kathrin ; Dugas, Martin ; Varghese, Julian ; Raab, Marc-Steffen ; Khandanpour, Cyrus: Inhibiting PI3K–AKT–mTOR Signaling in Multiple Myeloma-Associated Mesenchymal Stem Cells Impedes the Proliferation of Multiple Myeloma Cells
Inhalt
- 2 Methods
- 2.1 Sample Acquisition and Cell Culture
- 2.2 Cell Characterization
- 2.3 Cell Doubling Time
- 2.4 Assays to Measure the Influence of MSC and MM Cell Coculture
- 2.5 RNA Sequencing
- 2.6 Western Blot Analysis
- 2.7 Flow Cytometry Analysis for p-S6
- 2.8 Assays to Measure the Influence of Pictilisib on MSCs and MM Cell Lines
- 2.9 Statistical Analysis
- 3 Results
- 3.1 MM-Act-MSCs Supported the Growth of MM Cells More Than MM-Rm- and CTR-MSCs
- 3.2 MM-Act-MSCs Have a Distinct Gene Expression Profile Compared With CTR-MSCs
- 3.3 MM-Act-MSCs Showed Enriched Levels of Proteins Involved in PI3K–AKT–mTOR Signaling Compared With CTR-MSCs
- 3.4 MM-Act-MSCs Mediated the Antiproliferative Effect of Pictilisib on MM Cell Proliferation
- 4 Discussion
- Data Availability Statement
- Ethics Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References