Stolberg-Stolberg, Josef; Sambale, Meike; Hansen, Uwe; Schäfer, Alexandra; Raschke, Michael J.; Bertrand, Jessica; Pap, Thomas; Sherwood, Joanna: Cartilage Trauma Induces Necroptotic Chondrocyte Death and Expulsion of Cellular Contents
Inhalt
- Results
- Necroptotic Cell Death Can Be Induced In Vitro and Inhibited by Necrostatin-1
- Necroptotic Chondrocytes Contain Exocytotic Vesicles and Release Nucleic Acids Which Are Likely to Act as Ligands for Pattern Recognition Receptors
- Discussion
- Materials and Methods
- Human Cartilage Fragments
- Murine Cartilage Explants
- Immunohistochemistry
- Chondrocyte Isolation
- Induction of Necroptosis in In Vitro Chondrocytes
- Viability Assessment
- SDS-PAGE and Western Blotting
- Transmission Electron Microscopy
- PicoGreen Assay
- Statistics
- References