Spiegler, Verena: Anthelmintic A-Type Procyanidins and Further Characterization of the Phenolic Composition of a Root Extract from Paullinia pinnata
Inhalt
- Results
- Phytochemical Characterization of the Aqueous Acetone Root Extract
- Isolation and Identification of Oligomeric Proanthocyanidins
- Identification of Phytochemical Constituents Other than PACs
- Anthelmintic Activity of Extract and Isolated A-Type PACs
- Discussion
- Materials and Methods
- Plant Materials and Chemicals
- General Analytical Techniques
- Preparation of the Plant Extract
- Fractionation of the Acetone-Water (7:3) Extract
- Fractionation on Sephadex® LH20
- Fast Centrifugal Partition Chromatography
- Medium Pressure Liquid Chromatography (MPLC)
- Preparative TLC
- Preparative HPLC
- Hydrolysis of Glycosides
- Capillary Zone Electrophoresis (CZE)
- Identification of Isolated Compounds
- Caenorhabditis Elegans Culture and Mortality Assay
- Statistical Analysis
- Conclusions
- References